dopamine receptor d5 (d5r) antibody  (Santa Cruz Biotechnology)

Journal: CNS Neuroscience & Therapeutics

Article Title: Cortical dopamine D5 receptors regulate neuronal circuit oscillatory activity and memory in rats

doi: 10.1111/cns.14210

Figure Lengend Snippet: Knockdown of the D5R in PFC increases GSK‐3β activity. (A) The experimental timeline is shown. (B) Electrode placements in the PFC, OFC, thalamus, and HIP. (C) Representative images and quantification of fluorescence showing reduced PFC expression of the D5R (top panels) and GSK‐3β phosphorylation at Ser9 (bottom panels) following D5R‐shRNA induced knockdown. N = 7 rats/group (D) Images showing drd5 gene expression in control (left panels) animals or following drd5 mRNA knockdown (right panels). (E) Quantification of the number of drd5 mRNA expressing cells. N = 3 rats/group, 2 slices/rat. Quantified data are expressed as percent control. ** p < 0.01, *** p < 0.001 Student's t test.

Article Snippet: Subsequently, adjacent slices were incubated in primary antibodies rabbit anti‐pGSK‐3β (Ser9) (catalogue #ab9107166, 1:200, Abcam) or rabbit anti‐D5R (catalogue #ADR‐005, 1:200, Alomone Labs) for 60 h at 4°C.

Techniques: Knockdown, Activity Assay, Fluorescence, Expressing, shRNA, Control

Journal: CNS Neuroscience & Therapeutics

Article Title: Cortical dopamine D5 receptors regulate neuronal circuit oscillatory activity and memory in rats

doi: 10.1111/cns.14210

Figure Lengend Snippet: Effect of PFC D5R knockdown on oscillatory power activity in rats. Effect of PFC D5R knockdown on oscillatory power activity in rats. (A–D) Power spectra (left and center panels) and quantification of power (right panels) are shown. PFC D5R knockdown had the following regional effects on spectral power, (A) increased theta power in PFC, (B) increased theta power in OFC, and (C) increased theta power in HIP. (D) No effects of PFC D5R knockdown were evident in spectral power in the thalamus. Power curves are presented as normalized data with jackknife estimates of SEM shown as shaded areas. Quantified data are expressed as percent control. N = 7–8 rats/group, 1–2 electrodes/region/rat. * p < 0.05 Student's t test.

Article Snippet: Subsequently, adjacent slices were incubated in primary antibodies rabbit anti‐pGSK‐3β (Ser9) (catalogue #ab9107166, 1:200, Abcam) or rabbit anti‐D5R (catalogue #ADR‐005, 1:200, Alomone Labs) for 60 h at 4°C.

Techniques: Knockdown, Activity Assay, Control

Journal: CNS Neuroscience & Therapeutics

Article Title: Cortical dopamine D5 receptors regulate neuronal circuit oscillatory activity and memory in rats

doi: 10.1111/cns.14210

Figure Lengend Snippet: Effect of PFC D5R knockdown on oscillatory coherence in rats. (A–F) Coherence spectra (left panels) and quantification (right panels) are shown. (A) PFC D5R knockdown increased PFC‐OFC theta coherence, and (B) reduced PFC–thalamus high gamma coherence. (C–F) No significant effects on coherence were observed between PFC–HIP, OFC‐HIP, OFC‐thalamus, or thalamus‐HIP. Coherence curves are presented with jackknife estimates of SEM shown as shaded areas. Quantified data are expressed as percent control. N = 7–8 rats/group, 1–2 electrodes/region/rat. * p < 0.05, ** p < 0.01 Student's t test.

Article Snippet: Subsequently, adjacent slices were incubated in primary antibodies rabbit anti‐pGSK‐3β (Ser9) (catalogue #ab9107166, 1:200, Abcam) or rabbit anti‐D5R (catalogue #ADR‐005, 1:200, Alomone Labs) for 60 h at 4°C.

Techniques: Knockdown, Control

Journal: CNS Neuroscience & Therapeutics

Article Title: Cortical dopamine D5 receptors regulate neuronal circuit oscillatory activity and memory in rats

doi: 10.1111/cns.14210

Figure Lengend Snippet: D5R knockdown in PFC induces deficits in learning and memory. (A) D5R knockdown in PFC induced deficits in object recognition memory in the NOR test. (B) Impaired spatial memory in the OL test was also evident. (C) D5R knockdown also induced impairment in associative recognition memory when tested in the OiP. (D) In the Y‐maze, D5R knockdown had no effects on short‐term recognition memory (left panel) but resulted in impairment on long‐term memory (right panel). N = 7–8 rats/group, * p < 0.05, ** p < 0.01, *** p < 0.001 Student's t test.

Article Snippet: Subsequently, adjacent slices were incubated in primary antibodies rabbit anti‐pGSK‐3β (Ser9) (catalogue #ab9107166, 1:200, Abcam) or rabbit anti‐D5R (catalogue #ADR‐005, 1:200, Alomone Labs) for 60 h at 4°C.

Techniques: Knockdown

Journal: Frontiers in Neuroanatomy

Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

doi: 10.3389/fnana.2023.1111008

Figure Lengend Snippet: Information on primary antibodies.

Article Snippet: Dopamine receptor D5 (D5R) , C-terminal domain (aa 455–472) of rat D5R , Santa Cruz biotechnology; goat polyclonal; Cat# (R-18) sc-1441, RRID:AB_673640 , 1:300.

Techniques: Purification

Journal: Frontiers in Neuroanatomy

Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

doi: 10.3389/fnana.2023.1111008

Figure Lengend Snippet: Distribution of each dopamine receptor in the motor cortex and caudate putamen. (A) Representative immunohistochemistry of the frontal cortex for D1R. High-magnification images of rectangles indicated with E and I are shown in (E,I) . Moderate to strong D1R-immunoreactivities were observed in the pial surface of the cerebral cortex, layer (VI), and caudate putamen. (B) Representative immunohistochemistry of the frontal cortex for D2R. High magnification images of rectangles indicated with F and J are shown in (F) and (J) . (C) Representative immunohistochemistry of the frontal cortex for D4R. High magnification images of rectangles indicated with G and K are shown in (G) and (K) . (D) Representative immunohistochemistry of the frontal cortex for D5R. High magnification images of rectangles indicated with H and L are shown in (H) and (L) . (E–H) Immunohistochemical images of the cortical surface for D1R (E) , D2R (F) , D4R (G) , and D5R (H) . (I) Immunohistochemical images of the cortical layer V for D1R. Neuronal somata show weak immunopositive signals for D1R (arrowheads). (J) Immunohistochemical images of the cortical layer V for D2R. Neuronal somata show weak immunoreactivity for D2R (arrowheads). (K,L) Immunohistochemical images of the cortical layer V for D4R or D5R. Many neuronal somata (arrowheads) and apical dendrites (arrows) show immunoreactivity for D4R or D5R. Scale bars: 500 μm (A–D) ; 125 μm (E–H) ; 50 μm (I–L) .

Article Snippet: Dopamine receptor D5 (D5R) , C-terminal domain (aa 455–472) of rat D5R , Santa Cruz biotechnology; goat polyclonal; Cat# (R-18) sc-1441, RRID:AB_673640 , 1:300.

Techniques: Immunohistochemistry, Immunohistochemical staining

Journal: Frontiers in Neuroanatomy

Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

doi: 10.3389/fnana.2023.1111008

Figure Lengend Snippet: D5R-immunoreactivity in astrocytes. (A,B) Moderate D5R-immunoreactivities of the cortical surface of the prelimbic (A) and somatosensory (B) areas. (C,D) Confocal imaging of double immunostaining for D5R (magenta) and GS (green) indicated D5R-immunoreactivities biased toward the parenchymal side within pial astrocytes (arrowheads) in the prelimbic area. Processes of pial astrocytes (arrows) had only a few D5R-immunoreactivities. (E,F) D5R-immunoreactivities in the parenchymal side within pial astrocytes (arrowheads) in the somatosensory area. Processes of pial astrocytes (arrows) had only a few D5R-immunoreactivities. (G,H) In layer I of the primary motor area, astrocytes showed immunoreactivities for D5R (arrowheads). Strong and granular immunoreactivities for D5R were observed (magenta), which were in close vicinity of GS-positive structure (green), but not overlapped. (I,J) Confocal imaging of double immunostaining for D5R (magenta) and GS (green) indicated D5R-immunoreactivities in layer I astrocytes (arrows) in the primary motor area. (K,L) In layer I of the primary motor area, GS-positive astrocytes showed weak immunoreactivities for D5R (arrows). (M,N) In layer V of the primary motor area, GS-positive protoplasmic astrocytes (arrows) showed weak D5R-immunoreactivities. (O,P) In layer V of the primary motor area, strong D5R-immunoreactivities were observed in pyramidal cell-like large cells (asterisks) and their processes (arrows). Nuclei were stained with Hoechst33342. Scale bars: 20 μm (A,B) ; 10 μm (C–F,K–P) ; 5 μm (G–J) .

Article Snippet: Dopamine receptor D5 (D5R) , C-terminal domain (aa 455–472) of rat D5R , Santa Cruz biotechnology; goat polyclonal; Cat# (R-18) sc-1441, RRID:AB_673640 , 1:300.

Techniques: Imaging, Double Immunostaining, Staining

Journal: Frontiers in Neural Circuits

Article Title: Differential Expression of Dopamine D5 Receptors across Neuronal Subtypes in Macaque Frontal Eye Field

doi: 10.3389/fncir.2018.00012

Figure Lengend Snippet: Information on primary antibodies used for immunofluorescence.

Article Snippet: We performed two controls to verify the specifity of the D5R antibody from Alomone Labs (ADR-005, see Supplementary Figure ).

Techniques: Immunofluorescence

Journal: Frontiers in Neural Circuits

Article Title: Differential Expression of Dopamine D5 Receptors across Neuronal Subtypes in Macaque Frontal Eye Field

doi: 10.3389/fncir.2018.00012

Figure Lengend Snippet: Distribution of D5Rs across cortical layers. (A) Number of NeuN+ neurons that co-express D5R for cortical layers I, II–III, IV, V and VI per mm 2 . (B) Proportion of NeuN+ neurons that express D5Rs for cortical layers I, II–III, IV, V and VI.

Article Snippet: We performed two controls to verify the specifity of the D5R antibody from Alomone Labs (ADR-005, see Supplementary Figure ).

Techniques:

Journal: Frontiers in Neural Circuits

Article Title: Differential Expression of Dopamine D5 Receptors across Neuronal Subtypes in Macaque Frontal Eye Field

doi: 10.3389/fncir.2018.00012

Figure Lengend Snippet: D5R expression on different cell types. (A) Co-expression of D5Rs (green) and SMI-32+ putative long-range projection pyramidal neurons (red; left) and co-expression of D5Rs with a general pyramidal neuron stain (neurogranin, red; right). (B) Proportion of frontal eye field (FEF) SMI-32+ and Neurogranin+ neurons that express D5Rs. (C) Expression of D5Rs (green) among different interneuron subtypes (red): parvalbumin+ (top left), calretinin+ (top right), calbindin+ (bottom left), somatostatin+ (bottom right). (D) Proportion of inhibitory interneurons that express D5Rs. Statistical comparisons were made between parvalbumin+, calbindin+ and calretinin+ neurons. Somatostatin+ neurons were excluded because they were too sparse to be used in statistical comparisons. For all panels: scale bar is equal to 100 μm and *** denotes significance at the level p ≤ 0.001.

Article Snippet: We performed two controls to verify the specifity of the D5R antibody from Alomone Labs (ADR-005, see Supplementary Figure ).

Techniques: Expressing, Staining

Journal: Frontiers in Neural Circuits

Article Title: Differential Expression of Dopamine D5 Receptors across Neuronal Subtypes in Macaque Frontal Eye Field

doi: 10.3389/fncir.2018.00012

Figure Lengend Snippet: Proportion of D5Rs on different cell types across cortical layers. (A) Neurogranin+ pyramidal neurons and SMI-32+ putative long-range projection neurons. (B) Parvalbumin+, calbindin+, calretinin+ and somatostatin+ inhibitory interneurons. Proportions are broken down by cortical layer (I, II–III, IV, V and VI) and compared to SMI-32+ D5R+ proportions (light gray bars). *, ** and *** indicate significance at the levels of p ≤ 0.05, 0.01 and 0.001 (Bonferonni-adjusted values), respectively. Differences in the proportion of D5R+ neurons across layers were also calculated individually for each cell type. Only calretinin+ neurons exhibited a significant difference in D5R+ proportions across layers: indicated with a vertical line spanning layers II-VI and significance at the level of p ≤ 0.001 (Bonferonni-adjusted value) is indicated by ††† .

Article Snippet: We performed two controls to verify the specifity of the D5R antibody from Alomone Labs (ADR-005, see Supplementary Figure ).

Techniques:

Journal: Frontiers in Neural Circuits

Article Title: Differential Expression of Dopamine D5 Receptors across Neuronal Subtypes in Macaque Frontal Eye Field

doi: 10.3389/fncir.2018.00012

Figure Lengend Snippet: Proportion of D5R+ neurons by cell type for different cortical layers. For cortical layers I, II–III, IV, V and VI: proportion of D5R+ cells that express a given cell type marker (SMI-32, neurogranin, parvalbumin, calretinin, somatostatin).

Article Snippet: We performed two controls to verify the specifity of the D5R antibody from Alomone Labs (ADR-005, see Supplementary Figure ).

Techniques: Marker